Bio Enzyme Lab

Enzyme Lab Experiments Problem How can we demonstrate how enzymes work? What happens if we alter the surroundings of an enzyme? Materials Glucose Test StripsTest TubesPipettesRaw HamburgLettucePotato Raw LiverChalkBeakersDairy Lactose TabletWaterSugar Solo Cups Hot PlateKnifeGlovesSkim MilkGlow SticksPeroxide Hypothesis 1. If we change the environment via temperature the smooth pulsate forget Its enthusiasm will change 2. If hydrogen bleach is added to a certain food coloured then It would bubble 3. If a lactaid enzyme to draw the It would separate Procedure (A) 1. Collect three fire thumps. 2.Boil water in 400 mL beaker add 1st glowstick for 30 minutes. 3. Place molybdenum glowstick in freezer for 30 minutes 4. Leave third glow stick at board temperature for 30 minutes 5. hold open and record findings. information Collected (A) Place a glow stick in each environment below. Using a scale 1-3 1 being normal intensity 3 being brigh interrogatory intensity BeakerObservati on Freezer1 Boiling Water3 Room Temperature (control)2 Procedure (B) 1. Collect sample of rude(a) liver, potato, raw hamburg, lettuce and chalk. 2. Place each sample in individual running play vacuum underpass in rack. 3. Observe samples antecedent to adding peroxide. 4. channel 1 mL of peroxide to each test tube 5. Observe and record findings Data Collected (B) Reaction after hydrogen peroxide is added. Using a scale of 0-5 0 macrocosm no bubbles 5 being the most bubbles Test tubeObservation before adding hydrogen peroxideObservation after adding hydrogen peroxide 1 liverDark brown in color (expired meat)5 2 potatoInner slice with some skin2 3 raw hamburgNormal in color4 4 lettuceFresh green lettuce1 5 chalk Yellow colored chalk0 Procedure (C) Preparation 1. Enzyme Solution Add one lactase tablet to 200 ml of water. Stir until the tablet is dissolved. . Skim Milk This upshot contains lactose. 3. Sucrose SolutionAdd 5 grams of sugar to 100 ml of water. Stir until the sug ar is dissolved. 4. denaturised enzyme Solution 1. Place 20 ml of enzyme closure into a test tube. 2. Add 200 ml of water to a 400 ml beaker. 3. Place the test tube in the beaker. Make sure it does not spill out. 4. Place the beaker and the test tube on a hot plate. 5. Boil the water for 30 minutes. 6. Let the resultant cool to room temperature. Procedure (C-1) 1. Collect 6 mL of read milk, touch 2mL in each test tube (3 test tubes of skim milk). . Collect 4mL of saccharose firmness of purpose, place 2mL in each test tube (2 test tubes total). 3. Observe and record initial observations. 4. Add 1mL of enzyme solution to a skim milk test tube add 1mL of water to 2nd skim milk test tube, ad 1mL denatured enzyme solution to 3rd skim milk test tube. 5. Add 1mL of enzyme solution to 1st test tube of sucrose solution add 1mL of water to 2nd test tube of sucrose solution. 6. Insert Glucose test strip in wch test tube (5 total). Wait 2 minutes. 7. Observe and record whether or not gluc ose is present and how much.Test tubeInitial ObservationGlucose Test strip Is glucose present 1. 2 ml of skim milk and 1ml of the enzyme solutionBubbles3000 Glucose 2. 2 ml of skim milk and 1 ml of waterNo bubbles 2 layers300 Glucose 3. 2 ml of skim milk and 1 ml of denatured solutionBubbles and layers 0 Glucose 4. 2 ml of sucrose solution and 1 ml of enzyme solutionClear liquidYellow in color zero glucose 5. 2 ml of sucrose solution and 1 ml of waterClear liquid Yellow in color no glucose What happens when the enzyme is denatured? The Enzyme does not work. guide Questions 1. In which beaker did the glow stick glow the most?Why do you think that is? The beaker that contained hot water. The horniness from the water will speed up the chemical rate of reaction taking place, and therefore will glow brighter the cold one will use up its rate of reaction slowed, and thus be dimmer. 2. What did the glow stick show about enzymes and different environments? How does changing the temperatu re affect the rate of an enzyme-controlled experiment? The increasing temperature increases molecular(a) motion and may increase the number of times an enzyme contacts and combines with a substrate molecule.Temperature may also invite the shape of the enzyme molecule, making it fit better with the substrate. 3. What effect may change in PH have on an enzyme activity? The three-dimensional structure of a protein leaves certain side duress exposed. These side chains may attract ions from the environment. Under the right conditions, a group of positively charged hydrogen ions may accumulate on certain parts of an enzyme. A change in pH disrupts an enzymes shape and structure. When the pH changes an enzymes structure, the enzyme cant do its job. Changes in pH break the delicate bonds that maintain an enzymes shape.An enzyme will unravel, or denature, and become futile in a different pH. Stomach enzymes work in a super acidic environment of pH 2. A little way down the digestive tract , intestine enzymes need a pH of 8. 4. Why did the hamburger and liver react differently with hydrogen peroxide? They have natural catalase enzymes which were gloomy down with the presence of hydrogen peroxide. 5. What is the job of the lactase enzyme? Lactase is an enzyme which breaks down lactose, a sugar effect in milk and other dairy products. 6. In which test tubes was the glucose present? Why?In test tube 1 the enzyme broke down the lactose . Lactose is a disaccharide of glucose and galactose. The lactose tablet contained lactase which hydrolyzed the lactose into constituent galactose and glucose. Glucose is naturally occurring in skim milk thus in test tube 2 found in a much smaller amount than test tube 1. 7. What happened when the enzyme was denatured or altered by heat? Did it work the same? Was glucose present? There was no glucose present. It did not work at all. Summary What the class learn today about the jobs of enzymes? What questions does the class put away have ?